返回Google圖書搜尋

Survey of Non-histone Chromatin Proteins in a Differentiating Cell Line

其他書名	The 3T3-L1 Preadipocyte
出版	East Carolina University, 1984
URL	http://books.google.com.hk/books?id=rKuLtgAACAAJ&hl=&source=gbs_api

註釋Recent observations in several cell lines are consistent with the hypothesis that non-histone chromatin (NHC) proteins may be involved in the regulation of the differentiation process. Significant differences have been observed in populations of NHC proteins derived from cells prior to and after the onset of differentiation. This study utilizes the 3T3-L1 preadipocyte as a model system for looking at changes in NHC proteins during differentiation. NHC proteins were extracted and characterized using SDS-polyacrylamide gel electrophoresis. The characterization of NHC proteins obtained from undifferentiated and differentiated cells show that a variety of changes in the population of NHC proteins occurs during differentiation. Differentiation in these cells is accompanied by the appearance of proteins with molecular weights of 28,000, 30,000, 125,000, 150,000, and 200,000. A protein of 53,000 daltons was observed to vary in content with respect to time. Autoradiographic analysis of NHC proteins by densitometer scanning revealed that 11 proteins increase and 6 proteins decrease in amount when cells are induced to differentiate.