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Endothelial Cell Response to Microengineered Surfaces Analyzed by Fluorescent and Atomic Force Microscopy

出版	University of Florida, 2004
URL	http://books.google.com.hk/books?id=sZ_NjwEACAAJ&hl=&source=gbs_api

註釋ABSTRACT: Dysfunction of the endothelium leads to a number of cardiovascular diseases including atherosclerosis and intimal hyperplasia. As a first step toward engineering a synthetic small diameter vascular graft, the strategic use of micrometer scale topography and patterned chemistry has been used to control endothelial cell (EC) adhesion, spreading and overall function. Polystyrene (PS) Petri dishes and polydimethylsiloxane elastomer (PDMSe) were micropatterned with topographies and fibronectin (FN) using a combination of microprocessing and microcontact printing techniques. ECs were cultured for periods of 4, 7 and 14 days on the substrates and analyzed using immunoflourescent staining and atomic force microscopy (AFM). It was found that "1 micron vertical step heights in PDMSe disrupt EC adhesion and spreading for lateral spacings from 2 to 100 microns.