Date palm trees are exposed to infection with different diseases caused by many soil-borne pathogenic fungi that may cause considerable losses in the offshoots and trees. This study was carried out on five cvs. of date palm during two years in Aswan and New Valley governorates. Isolation and identification of associated fungi showed that the most frequent fungi were Fusarium oxysporum, F. moniliforme and Thielaviopsis paradoxa while the least frequent was Phytophthora sp. The cultivars were differed susceptible to infection by the isolated pathogenic fungi. Barhey cultivar was the most susceptible to infection, followed by Gondaila, Siwey and Partmoda cultivars, while Sakkoty cultivar was the least susceptible. Seven streptomycetes were isolated from the rhizosphere soil of healthy date palm cultivated in Aswan governorate. Streptomycetes were namated viz. Streptomyces bobilii (isolate A1, A5 and A7), Streptomyces grisiobrunneus (isolate A2 and A3), Streptomyces albolongus (isolate A4 and A6). Three isolates A1, A6 and A3 of them gave highest results in vitro and in greenhouse experiments against soil-borne pathogenic fungi compared with negative and positive control. The obtained results showed that Streptomyces had the highest efficacy on root rots of date palm. This is the first study done to use actinomycetes against soil-borne pathogenic fungi that caused root rots of date palm.