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Making a Chromosomal Assignment for the Juvenile Alopecia (jal) Mutation in Mice

出版	Central Connecticut State University, 2012
URL	http://books.google.com.hk/books?id=umO9MwEACAAJ&hl=&source=gbs_api

註釋The juvenile alopecia (jal) mutation in mice arose on the standard C3H/HeJ inbred background. This mutation controls a poorly-understood recessive disorder in which affected subjects display patchy hair loss at an early age, often retaining only small patches of fur. The site disrupted by this mutation is unknown, although a previous research publication (McElwee et al. 1999) attributed the mutation to mouse Chromosome (Chr) 13 without providing any supporting data. Here I aimed to verify and refine this chromosomal assignment for jal, using a standard intraspecific backcross mapping approach. To genetically map jal, I created an F1 generation by crossing C57BL/6J female mice with C3H/HeJ males that are homozygous for jal. The heterozygous females were backcrossed to C3H-jal/jal males to make an N2 generation that included 103 members. These mice were classified for their fur phenotype and typed for previously-mapped, PCR-scorable DNA markers. Independent assortment of jal and a particular DNA marker indicated that jal is not located near that site. Since my early analysis using markers from Chr 13 showed that jal is not located on Chr 13, I also assessed numerous markers from throughout the mouse genome for possible linkage with jal. While most sites tested assorted independently with jal, I finally detected linkage at the centromeric tip of mouse Chr 2. Because this region of the C57BL/6J strain offers very few usable markers to support further map refinement, I recommend that a new backcross be conducted using a partner strain that carries numerous molecular differences compared with the C3H/HeJ strain in this region.