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Residues of MS-222, Benzocaine, and Their Metabolites in Striped Bass Following Anesthesia

出版	U.S. Department of the Interior, Fish and Wildlife Service, Bureau of Sport Fisheries and Wildlife, 1973
URL	http://books.google.com.hk/books?id=vu4QnQEACAAJ&hl=&source=gbs_api

註釋Striped bass anesthetized in a 100-mg/liter solution of MS-222 at 17.5 degrees Centigrade contained an average of 57.9 microgram/gram of MS-222 and 23.3 microgram/gram of m-aminobenzoic acid residues in the muscle tissue immediately after a 30-minute exposure to the drug (0-hour withdrawal samples). After this tissue was homogenized and stored in a refrigerator for 1 week at 1.7 degrees Centigrade, residues were 100 percent m-aminobenzoic acid. Fish anesthetized with benzocaine were treated in a like manner still contained residues of benzocaine and a small amount of p-aminobenzoic acid (3.4 percent) after storage. The ester and acid residues of both anesthetics decreased steadily with length of recovery time. The residues were measured by a modified Bratton-Marshall colorimetric method and confirmed by thin-layer chromatography.