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Cellular Adhesion of Normal and Cancerous Colon Cells

其他書名	Effects of Surface Topography
出版	University of Florida, 2007
URL	http://books.google.com.hk/books?id=wD-OAQAACAAJ&hl=&source=gbs_api

註釋ABSTRACT: Normal cellular adhesion is a key factor to maintain healthy human biological functions. Adhesion processes are influenced by many factors such as detachment forces and extra cellular topography, but the mechanisms involved are not well understood because of a lack of well-defined, simple, experimental systems for long-term cell adhesion. The literature also suggests that in the context of cancerous organs, an altered cellular adhesion can promote the spread of metastases. Therefore, in order to better characterize and gain new insights into the effects of some key mechanical factors, we have developed several simple, easy to use, techniques for evaluating the roles of both normal and shear forces, and surface roughness in cell adhesion, with a focus on cancer cells. The results show as expected that, as the flow rate or the normal force is increased, more cells are detached from the surface substrate. However, it is found that, for a smooth surface, 20% of the healthy cells will detach at the onset of an applied normal force, and that the rate of detachment is negligible after that. In contrast, cancer cells continuously detach as the normal force is increased. Another difference seen between healthy and cancer cells is in the behavior of doublet cells. Healthy doublets have a much stronger adhesion than single cells. However, this difference is not seen with cancer cells. Finally, it is observed that surface roughness produces different effects on the adhesion of healthy and cancerous colon cells.