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Advanced Techniques in Plant Sciences
Dr. D. A. Dhale
出版Book Saga Publications , 2023-05-28
主題Education / Teaching / Subjects / Science & TechnologyScience / Biotechnology
ISBN93954704299789395470421
URLhttp://books.google.com.hk/books?id=wzPBEAAAQBAJ&hl=&source=gbs_api
EBookSAMPLE
註釋This book, ‘Advanced Techniques in Plant Sciences’ includes basic and advanced techniques like microscopy, polarizing microscopy, transmission and scanning electron microscopes, confocal microscopy, phase contrast microscopy, photomicrography, digital imaging, and microscopic measurements. The Spectroscopic techniques contain Colorimeter, Spectroscopic techniques, Ultraviolet-visible spectroscopy; Infrared spectroscopy, Nuclear magnetic resonance spectroscopy; Basic NMR techniques, NMR Imaging Principles, Electron Spin Resonance (ESR) Spectroscopy, Atomic Absorption Spectroscopy (AAS); Mass spectrometry, Flame photometer, etc. In histochemical studies, various methods include proteins, carbohydrates, lipids, and enzymes; how to prepare the macrolides; and the different methods of preparing the slides, which include Along with this, you will also study whole mounts. Also, about the sectioning of plant specimens, free hand, microtome, rotary microtome sectioning, types of microtome, and methods of serial sectioning. Maceration, Gel electrophoresis, PAGE, SDS-PAGE, Agarose gel electrophoresis; isoelectric focusing, and 2D electrophoresis Blotting Techniques: Principles and Techniques of Southern, Northern, and Western Blotting Techniques and Southwestern Blotting. Centrifugation techniques, Types of Centrifugation, Ultracentrifugation, Concept of Partition Coefficient; Chromatography, Techniques by chromatographic bed shape, Paper Chromatography, Double-way paper chromatography, Planar chromatography, Thin layer chromatography (TLC), High-performance liquid chromatography (HPLC)- Partition chromatography, Normal-phase chromatography, Displacement chromatography, Reversed-phase chromatography (RPC), Size-exclusion chromatography, Ion-exchange chromatography, Bioaffinity chromatography, Aqueous normal-phase chromatography, Isocratic flow, and gradient elution; High-Performance Thin-Layer Chromatography (HPTLC), Gas chromatography (GC), Flash column chromatography, Column chromatography, Ion chromatography, Gel-filtration chromatography or Size-exclusion chromatography, Pyrolysis gas chromatography, Countercurrent chromatography.