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Parentage-based Tagging of Snake River Hatchery Steelhead and Chinook Salmon
其他書名
Project Progress Report, 2020 Annual Report
出版Idaho Fish & Game, 2021
URLhttp://books.google.com.hk/books?id=y_fmzgEACAAJ&hl=&source=gbs_api
註釋This report summarizes the continued development and evaluation of a genetic technology called Parentage-Based Tagging (PBT), a versatile tool for genetically tagging steelhead Oncorhynchus mykiss and Chinook Salmon O. tshawytscha in the Snake River basin. While PBT is potentially a more economical and efficient technique for tagging fish than coded wire tags (CWT), it also has the capability to address aspects of hatchery practices, salmonid life history, harvest patterns, and trait heritability. This report summarizes three objectives for this performance period of January 1, 2020 to December 31, 2020 that focused on the continued development and implementation of PBT in the Snake River basin: Objective 1) annual sampling of hatchery broodstock, Objective 2) creation of genetic parental databases, and Objective 3) utilization of PBT to provide parentage assignments for hatchery fish of unknown origin. This project continues to sample and inventory all hatchery broodstock (Objective 1) for steelhead (~5,000 individuals annually), spring/summer Chinook Salmon (~10,000 individuals annually), and fall Chinook Salmon (~2,500) in the Snake River basin. In close collaboration with the Columbia River Inter-Tribal Fisheries Commission (CRITFC), we used a highly variable panel of single nucleotide polymorphism (SNPs) markers, identified for each species, to genotype steelhead and Chinook Salmon broodstocks sampled in the Snake River basin from spawn year (SY) 2019 (Objective 2). We then used the data generated from the broodstock baselines to provide parentage results to inform a variety of management and conservation programs (Objective 3). Results continued to indicate that annual sampling, inventorying, and genotyping of all steelhead and Chinook Salmon broodstock in the Snake River basin is feasible and that the SNP sets identified for PBT are sufficient for accurate assignment of offspring to brood year and hatchery stock, thereby allowing an unprecedented ability to mark millions of hatchery-origin fish from the Snake River and an opportunity to address future objectives of parentage-based management.