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Glycomics
Sureyya Ozcan
其他書名
Applications to Clinical Studies
出版University of California, Davis, 2012
ISBN12679693509781267969354
URLhttp://books.google.com.hk/books?id=z0n4sgEACAAJ&hl=&source=gbs_api
註釋Glycosylation is one of the most important post-translational modifications (PTM) indicating decorations of proteins by attachment of sugar residues. Over fifty years of research on glycans demonstrates the importance of these sugar molecules in a variety of disciplines including clinical science and pharmaceutical researches. Human glycoproteins are becoming promising targets for disease research because they play an essential role in many biological processes, including immune defense, cell growth, and cell-cell adhesion. Research shows that glycans on glycoproteins are active participants in protein stability, cell-cell interactions, and cell development. Moreover protein glycosylation in human serum is known to change with disease, pregnancy, age, and inflammation. Thus, understanding of the human glycome is very important to understand the biological processes behind disease formation, progression and treatment. Our study is based on the fact that glycosylation is highly sensitive to the biochemical environment and has been implicated in many diseases. Firstly, glycomics approach was used to find glycan markers for ocular rosacea, a chronic dry eye disease. The glycomic analysis of tears and saliva of roseatic patients and comparison to normal patients were performed in order to identify potential oligosaccharide markers for rosacea and to make an early and specific diagnosis possible. Subsequently, proposed glycan markers for ocular rosacea study were further characterized by Mass Spectrometry (MS) based techniques. Several, unique glycan structures were elucidated based on Tandem MS. Clinical studies were followed by monitoring serum glycans isolated from gastric, ovarian and prostate cancer patients. Our results showed promising glycan markers for each disease category, and performance of the candidate glycans was tested by ANOVA based statistical studies. Furthermore, the analytical method was modified to improve sensitivity. The presented study will lead to future studies using glycomics as a tool for understanding health and disease progress.