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Genetic Analysis of 15 STR Loci of Chinese Uigur Ethnic Population
B. Zhu
Z. Wang
Q. Wu
M. Eli Sedike
J. Zhu
P. Huang
Y. Xu
Y. Liu
出版
ASTM International
, 2005
URL
http://books.google.com.hk/books?id=zXl3zgEACAAJ&hl=&source=gbs_api
註釋
The blood samples were obtained from 106 unrelated healthy individuals from Yili Uigur ethnic autonomous region, Xin Jiang Province of China. Genomic DNA was extracted using the Chelex100 protocol as described by Walsh et al. (1) PCR for 15 STRs was performed in multiplex reaction using AmpFLSTR Identifiler kit; 0.9 μL (2 ng/μL) genomic DNA samples were amplified in a total reaction volume of 10 μL along with 2.9 μL deionized water, 4 μL dNTP, 0.2 μL AmpliTaqGold DNA polymerase, and 2.0 μL primer set. Thermal cycling was conducted with the below conditions: 95°C for 11 min; 28 cycles of 94°C for 60 sec, 59°C for 60 sec, 72°C for 60 sec; and a final extension of 60°C for 45min. Detection and genotyping of all PCR products were accomplished using ABI3100 DNA Genetic Analyzer (Applied Biosystem) Allele designation was done using GeneScan3.7 and Genotyper3.7.
