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Actinomycetes tyrosinase
Dr. Shivaveerakumar S.
出版Xoffencerpublication, 2022-01-01
主題Political Science / Public Policy / Agriculture & Food Policy
ISBN93947072639789394707269
URLhttp://books.google.com.hk/books?id=zno9EQAAQBAJ&hl=&source=gbs_api
EBookSAMPLE
註釋Actinomycetes are Gram-positive bacteria with high G+C content and are abundant in soil. They use an extraordinary variety of extracellular mechanisms for primary growth and to facilitate intimate associations with other organisms. The large genome size (of about 8 Mb) of Streptomyces make it quite interesting and attractive towards high metabolic diversity. Tyrosinase catalyses the first two reactions: the conversion of tyrosine to dihydroxyphenylalanine (dopa) and the oxidation of dopa to o-dopaquinone. It is found in both prokaryotes and eukaryotic microbes, invertebrates, plants and mammals. Metagenomics is the application of modern genomics techniques to the study of communities of microbial organisms directly in their natural environments. Enzyme purification is a series of processes intended to isolate a single type of protein from a complex mixture. Purification methods were slightly modified depending on their origin or source. Tyrosinase was purified from various sources, such as plants, mammals and microorganisms. An industrial disadvantage of the most commercially used enzymes and enzyme complexes - is their relatively low stability. The most important applications of tyrosinases are the biosynthesis of an intermediate compound L-DOPA. It has been employed as biosensor for the detection of various phenolic constituents from polluted soils and water samples. Antioxidants terminate chain reactions by removing free radical intermediates, and inhibit other oxidation reactions.