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Protocols on Algal and Cyanobacterial Research
S. N. Bagchi
Diethelm Kleiner
Prasanna Mohanty
出版
Alpha Science
, 2010
主題
Science / Life Sciences / Biochemistry
Science / Life Sciences / Botany
Science / Life Sciences / Marine Biology
Science / Life Sciences / Microbiology
Technology & Engineering / Materials Science / General
ISBN
1842655841
9781842655849
URL
http://books.google.com.hk/books?id=zpKPSQAACAAJ&hl=&source=gbs_api
註釋
This is an up-to-date and consolidated compilation of ready-to-use protocols embodied in twenty three chapters and grouped in four sections, highlighting methods related to preparation and characterization of components, assessment of biological activities of toxins, and biotechnology utilizing algae and cyanobacteria as research material.
In the first section aspects of sub-cellular fractionation and estimation of photosynthetically active particles, phycobilisomes, carotenoids, lipids, DNA, RNA, proteins and peptides are discussed. The second section is devoted to the analysis of physiological processes namely transport and assimilation of ammonia, phosphate, nitrate and water (Solutes), and primary photochemical activity and CO2 fixation. A variety of toxins of planktonic algae affect the ecosystem balance and human/animal life and the third section illustrates the procedures for their determination. Since microalgae can sense and respond to toxic anthropogenic compounds like heavy metals, the application of various indicatory/sequestering bioassays is the highlight of the fourth section.