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In Toto Light Sheet Fluorescence Microscopy Live Imaging Datasets of Ceratitis Capitata Embryonic Development

Frederic StroblMarc Florian ScheteligErnst H. K. Stelzer

出版	Nature Publ. Group, 2022
URL	http://books.google.com.hk/books?id=VQ0G0AEACAAJ&hl=&source=gbs_api

註釋The Mediterranean fruit fly (medfly), Ceratitis capitata, is an important model organism in biology and agricultural research with high economic relevance. However, information about its embryonic development is still sparse. We share nine long-term live imaging datasets acquired with light sheet fluorescence microscopy (484.5)h total recording time, 373 995 images, 256)Gb) with the scientific community. Six datasets show the embryonic development in toto for about 60)hours at 30)minutes intervals along four directions in three spatial dimensions, covering approximately 97% of the entire embryonic development period. Three datasets focus on germ cell formation and head involution. All imaged embryos hatched morphologically intact. Based on these data, we suggest a two-level staging system that functions as a morphogenetic framework for upcoming studies on medfly. Our data supports research on wild-type or aberrant morphogenesis, quantitative analyses, comparative approaches to insect development as well as studies related to pest control. Further, they can be used to test advanced image processing approaches or to train machine learning algorithms and/or neuronal networks.